Coding
HMG-CoA-R

Part:BBa_K849000:Design

Designed by: Leonard Sebastian Fresenborg   Group: iGEM12_Frankfurt   (2012-09-20)

3-Hydroxy-3-Methyl-Glutaryl-CoA-Reductase from Yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 685
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1456
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The truncation was done according to the following publication:

[http://aem.asm.org/content/76/19/6449.short|Rico, Pardo, Orejas: 'Enhanced Production of a Plant Monoterpene by Overexpression of the 3-Hydroxy-3-Methylglutaryl Conenzyme A Reductase Catalytic Domain in Saccharomyces cerevisiae'; Applied and Enviromental Microbiology; Vol 76 p. 6449-6454; 2010]

Source

The original source of this part is the genome of Saccharomyces cerevisiae. We obtained the sequence by de novo synthesis. For that we used data from the [http://www.yeastgenome.org Saccharomyes Genome Database]. We inserted some calm mutations to avoid conflicts with the Cloning Standard 10.

References